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The microbiome of diabetic foot osteomyelitis.
van Asten SA, La Fontaine J, Peters EJ, Bhavan K, Kim PJ, Lavery LA
Submitted Externally on 5/25/2016
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Volume : Pages
35 : 293 - 8
The purpose of this investigation was to evaluate the diversity of bacteria in
diabetic foot osteomyelitis using a 16S rRNA sequencing approach and to compare
the results with conventional culture techniques. In this prospective
observational study, we obtained 34 bone samples from patients admitted to our
hospital with a moderate-severe diabetic foot infection. We analysed the
distribution of the 16S rRNA gene sequences in the bone samples, using an
Illumina MiSeq Personal Sequencer. We compared the genera that were detected
with the cultured pathogens in the bone samples with conventional techniques. In
the 23 samples that had positive results with both techniques, Staphylococcus,
Corynebacterium, Streptococcus and Propionibacterium spp. were detected in 20,
18, 13 and 11 samples, respectively. Significantly more anaerobes were detected
with 16S rRNA sequencing compared to conventional techniques (86.9 % vs. 23.1 %,
p?=?0.001) and more Gram-positive bacilli were present (78.3 % vs. 3.8 %,
p?0.001). Staphylococcus spp. were identified in all of the sequenced bone
samples that were negative with conventional techniques. Mixed genera were
present in 83.3 % (5 of 6) of the negative samples. Anaerobic and fastidious
organisms may play a more significant role in osteomyelitis than previously
reported. Further studies with larger populations are needed in order to fully
understand the clinical importance of the microbial diversity of diabetic foot
Neuropathy & Neurocognition
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Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
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