The microbiome of diabetic foot osteomyelitis.
Authors van Asten SA, La Fontaine J, Peters EJ, Bhavan K, Kim PJ, Lavery LA
Submitted By Submitted Externally on 5/25/2016
Status Published
Journal European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Year 2016
Date Published 2/1/2016
Volume : Pages 35 : 293 - 8
PubMed Reference 26670675
Abstract The purpose of this investigation was to evaluate the diversity of bacteria in
diabetic foot osteomyelitis using a 16S rRNA sequencing approach and to compare
the results with conventional culture techniques. In this prospective
observational study, we obtained 34 bone samples from patients admitted to our
hospital with a moderate-severe diabetic foot infection. We analysed the
distribution of the 16S rRNA gene sequences in the bone samples, using an
Illumina MiSeq Personal Sequencer. We compared the genera that were detected
with the cultured pathogens in the bone samples with conventional techniques. In
the 23 samples that had positive results with both techniques, Staphylococcus,
Corynebacterium, Streptococcus and Propionibacterium spp. were detected in 20,
18, 13 and 11 samples, respectively. Significantly more anaerobes were detected
with 16S rRNA sequencing compared to conventional techniques (86.9 % vs. 23.1 %,
p?=?0.001) and more Gram-positive bacilli were present (78.3 % vs. 3.8 %,
p?samples that were negative with conventional techniques. Mixed genera were
present in 83.3 % (5 of 6) of the negative samples. Anaerobic and fastidious
organisms may play a more significant role in osteomyelitis than previously
reported. Further studies with larger populations are needed in order to fully
understand the clinical importance of the microbial diversity of diabetic foot