Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and
translational implications.
Authors Winfree S, Ferkowicz MJ, Dagher PC, Kelly KJ, Eadon MT, Sutton TA, Markel TA,
Yoder MC, Dunn KW, El-Achkar TM
Submitted By Submitted Externally on 9/19/2017
Status Published
Journal Translational research : the journal of laboratory and clinical medicine
Year 2017
Date Published 7/1/2017
Volume : Pages Not Specified : Not Specified
PubMed Reference 28784428
Abstract Recent developments in automated optical sectioning microscope systems have
enabled researchers to conduct high resolution, three-dimensional (3D)
microscopy at the scale of millimeters in various types of tissues. This
powerful technology allows the exploration of tissues at an unprecedented level
of detail, while preserving the spatial context. By doing so, such technology
will also enable researchers to explore cellular and molecular signatures within
tissue and correlate with disease course. This will allow an improved
understanding of pathophysiology and facilitate a precision medicine approach to
assess the response to treatment. The ability to perform large-scale imaging in
3D cannot be realized without the widespread availability of accessible
quantitative analysis. In this review, we will outline recent advances in
large-scale 3D imaging and discuss the available methodologies to perform
meaningful analysis and potential applications in translational research.