Endothelial cell crosstalk improves browning but hinders white adipocyte
maturation in 3D engineered adipose tissue.
Authors Hammel JH, Bellas E
Submitted By Submitted Externally on 4/28/2020
Status Published
Journal Integrative biology : quantitative biosciences from nano to macro
Year 2020
Date Published 4/1/2020
Volume : Pages 12 : 81 - 89
PubMed Reference 32219324
Abstract Central to the development of adipose tissue (AT) engineered models is the
supporting vasculature. It is a key part of AT function and long-term
maintenance, but the crosstalk between adipocytes and endothelial cells is not
well understood. Here, we directly co-culture the two cell types at varying
ratios in a 3D Type I collagen gel. Constructs were evaluated for adipocyte
maturation and function and vascular network organization. Further, these
constructs were treated with forskolin, a beta-adrenergic agonist, to stimulate
lipolysis and browning. Adipocytes in co-cultures were found to be less mature
than an adipocyte-only control, shown by smaller lipid droplets and
downregulation of key adipocyte-related genes. The most extensive vascular
network formation was found in the 1:1 co-culture, supported by vascular
endothelial growth factor (VEGF) upregulation. After forskolin treatment, the
presence of endothelial cells was shown to upregulate PPAR coactivator 1 alpha
(PGC-1a) and leptin, but not uncoupling protein 1 (UCP1), suggesting a specific
crosstalk that enhances early stages of browning.