De novo production of human extracellular matrix supports increased throughput
and cellular complexity in 3D skin equivalent model.
Authors Smith A, Huang M, Watkins T, Burguin F, Baskin J, Garlick JA
Submitted By Submitted Externally on 7/20/2020
Status Published
Journal Journal of tissue engineering and regenerative medicine, REFERENCES
Year 2020
Date Published 6/1/2020
Volume : Pages Not Specified : Not Specified
PubMed Reference 32483913
Abstract Three-dimensional (3D) tissue models of human skin are being developed to better
understand disease phenotypes and to screen new drugs for potential therapies.
Several factors will increase the value of these in vitro 3D skin tissues for
these purposes. These include the need for human-derived extracellular matrix
(ECM), higher throughput tissue formats, and greater cellular complexity. Here,
we present an approach for the fabrication of 3D skin-like tissues as a platform
that addresses these three considerations. We demonstrate that human adult and
neonatal fibroblasts deposit an endogenous ECM de novo that serves as an
effective stroma for full epithelial tissue development and differentiation. We
have miniaturized these tissues to a 24-well format to adapt them for eventual
higher throughput drug screening. We have shown that monocytes from the
peripheral blood can be incorporated into this model as macrophages to increase
tissue complexity. This humanized skin-like tissue decreases dependency on
animal-derived ECM while increasing cellular complexity that can enable
screening inflammatory responses in tissue models of human skin.