Feature-rich covalent stains for super-resolution and cleared tissue
fluorescence microscopy.
Authors Mao C, Lee MY, Jhan JR, Halpern AR, Woodworth MA, Glaser AK, Chozinski TJ, Shin
L, Pippin JW, Shankland SJ, Liu JTC, Vaughan JC
Submitted By Submitted Externally on 8/11/2020
Status Published
Journal Science advances
Year 2020
Date Published 5/1/2020
Volume : Pages 6 : eaba4542
PubMed Reference 32518827
Abstract Fluorescence microscopy is a workhorse tool in biomedical imaging but often
poses substantial challenges to practitioners in achieving bright or uniform
labeling. In addition, while antibodies are effective specific labels, their
reproducibility is often inconsistent, and they are difficult to use when
staining thick specimens. We report the use of conventional, commercially
available fluorescent dyes for rapid and intense covalent labeling of proteins
and carbohydrates in super-resolution (expansion) microscopy and cleared tissue
microscopy. This approach, which we refer to as Fluorescent Labeling of Abundant
Reactive Entities (FLARE), produces simple and robust stains that are modern
equivalents of classic small-molecule histology stains. It efficiently reveals a
wealth of key landmarks in cells and tissues under different fixation or sample
processing conditions and is compatible with immunolabeling of proteins and in
situ hybridization labeling of nucleic acids.

Complications