Sign-up for our newsletter
MAIN
Event Calendar
Awardee Reports
ABOUT DIACOMP
Citing DiaComp
Contact
Committees
Institutions
Awardee Reports
Funding Programs
Pilot & Feasibility
Conference Support
Summer Student
Collaborative
External Funding Programs
Publications
Bioinformatics
RESOURCES
Protocols & Methods
Reagents & Resources
Mouse Diet
Breeding Schemes
Validation Criteria
IMPC / KOMP Data
Publications
Bioinformatics
FUNDING PROGRAMS
Pilot & Feasibility
Conference Support
Summer Student
Collaborative
External Funding Programs
CONTACT
PARTICIPANT AREA
Login
Request Account
▹
DiaComp Funded Abstracts
Program Application Abstract
Development of an RNA-FISH Procedure for Detection of mRNA Targets in Diabetic Nephropathy
Schulte, Emma
(University of Washington)
Mentor: Akilesh, Shreeram
Summer Student Program
Diabetic nephropathy (DN) is a common complication of diabetes and is associated with significant morbidity and mortality among patients. As the most common cause of end-stage renal disease (ESRD) in the United States, DN remains a significant burden to national public health. While the glomerulus, a key structure in the kidney filter, is the principal target of diabetic kidney injury, there are currently no approved treatments that reverse the glomerular injury seen in DN. Our lab has used modern genetic approaches to identify novel glomerular target genes for DN and other kidney diseases (PMID: 30760496). As a next step, precise localization of target gene expression in key cell types within the tissue context will be essential to understand their mechanistic role in DN. In situ detection of messenger RNA (mRNA) molecules using hybridization of fluorescently labeled probes (RNA-FISH) is the most powerful and versatile technique to accomplish this, but is technically challenging. In order to establish this technique within our laboratory, we tested cells grown in vitro, and mouse and human kidney tissues that were preserved using different strategies. We initially targeted detection of polyadenylated messenger RNA (mRNA) using a fluorescently labeled oligo-dT50 probe as a test for RNA integrity and imaged our preparations on both widefield and confocal microscopes. While the initial experiments appear to have been unsuccessful, we will next focus on optimizing tissue preservation, target selection and imaging to bring this powerful methodology to bear on the study of novel glomerular targets in DN.
Welcome to the DiaComp Login / Account Request Page.
Email Address:
Password:
Note: Passwords are case-sensitive.
Please save my Email Address on this machine.
Not a member?
If you are a funded DiaComp investigator, a member of an investigator's lab,
or an external advisor to the consortium, please
request an account.
Forgot your password?
Enter your Email Address and
click here.
ERROR!
There was a problem with the page:
User Info
User Confirm
Safari Browser Detected...
We strive to make the DiaComp site compatable with as many browsers as possible, but some of our third party tools don't work with the Safari browser.
In order to explore this site we highly recommend using the most recent versions of the following browsers:
Internet Explorer
Google Chrome
FireFox
Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
Citation text and image have been copied to your clipboard. You may now paste them into your document. Thank you!