Alterations in neurogenically mediated contractile responses of urinary bladder
in rats with diabetes.
Authors Liu G, Daneshgari F
Submitted By Firouz Daneshgari on 2/4/2009
Status Published
Journal American journal of physiology. Renal physiology
Year 2005
Date Published 6/1/2005
Volume : Pages 288 : F1220 - F1226
PubMed Reference 15687244
Abstract Diabetic bladder dysfunction (DBD) is among the most common and bothersome
complications of diabetes mellitus. Autonomic neuropathy has been counted as the
cause of DBD. In the present study, we compared the alterations in the
neurogenically mediated contractile responses of urinary bladder in rats with
streptozocin-induced diabetes, 5% sucrose-induced diuresis, and age-matched
controls. Male Sprague-Dawley rats were divided into three groups: 9-wk diabetic
rats, diuretic rats, and age-matched controls. Micturition and morphometric
characteristics were evaluated using metabolic cage and gross examination of the
bladder. Bladder detrusor muscle strips were exposed to either periodic
electrical field stimulation (EFS) or to EFS in the presence of atropine,
alpha,beta-methylene adrenasine 5'-triphosphate, or tetrodotoxin. The
proportions of cholinergic, purinergic, and residual
nonadrenergic-noncholinergic (NANC) components of contractile response were
compared among the three groups of animals. Diabetes caused a significant
reduction of body weight compared with diuresis and controls, although the
bladders of diabetic and diuretic rats weighed more than the controls. Both
diabetes and diuresis caused significant increase in fluid intake, urine output,
and bladder size. Diabetes and diuresis caused similarly increased response to
EFS and reduced response to cholinergic component compared with controls.
However, the purinergic response was significantly smaller in diuretic bladder
strips compared with controls but not in diabetic rats. A residual NANC of
unknown origin increased significantly but differently in diabetics and
diuretics compared with controls. In conclusion, neurogenically mediated bladder
contraction is altered in the diabetic rat. Diabetic-related changes do not
parallel diuretic-induced changes, indicating that the pathogenesis of DBD needs
further exploration.

Investigators with authorship
Firouz DaneshgariCase Western Reserve