DiaComp :: Protocols

The following protocols are used by the DiaComp. Click the title for details, or download the protocol in PDF format (if available).

Complications: (Click an image below to filter by diabetic complication)

Cardiovascular

Neuropathy

Wound-Healing

Gastro-Intestinal (GI)

Pediatric Endocrinology

Retinopathy

Nephropathy

Uropathy

All Complications

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Breeding	Contact	Complication
Breeding NONcNZO10/LtJ (NON/NZ) and NON/ShiLtJ (NON/Shi) mice	Simpson, Ian
Breeding Scheme and Selection of Animals for DCC Experiments	Maeda, Nobuyo Smithies, Oliver Takahashi, Nobuyuki

Complication Induction	Contact	Complication
Induction of Type 1 Diabetes, Myocardial Infarction, Echocardiography, Measurement of Blood Glucose and Tissue Weights	Hill, Michael

Diabetes Induction	Contact	Complication
Ablation of ß-cells in Tg(ins:NTR-mCherry) transgenic zebrafish using metronidazole	Rieger, Sandra
Dechorionation of zebrafish embryos with Pronase for metronidazole-mediated ß-cell ablation	Rieger, Sandra
High Dose STZ Induction Protocol	Brosius, Frank
Low-Dose Streptozotocin Induction Protocol (mouse)	Brosius, Frank
Mouse Diets Used in Diabetic Complication Research	McIndoe, Richard

Dissection	Contact	Complication
Isolation of rodent eyes for analysis of diabetic retinopathy	Kern, Tim
Removal and Preservation of the Urinary Bladder	Daneshgari, Firouz

Histology	Contact	Complication
En face preparation and quantification of aortic surface area covered by atherosclerotic lesions	Breslow, Jan
Mouse dissection and preparation of heart (aortic sinus) and brachiocephalic artery for lesion quantitation by sectioning	Breslow, Jan

PCR Amplification	Contact	Complication
Quantitative PCR analysis to assess gene expression changes in hyperglycemic larval zebrafish	Rieger, Sandra

Phenotype Assay	Contact	Complication
Aortic Banding in Mice	Abel, E. Dale
Atherosclerotic Morphometry in Pigs	Clemmons, David Nichols, Timothy
Cardiac AMPK Assay	Abel, E. Dale
CATALASE	Brosius, Frank Feldman, Eva
Confocal imaging of live larval zebrafish for assessing peripheral neuropathy	Rieger, Sandra
Conscious Cystometry Bladder Function Testing	Daneshgari, Firouz
Creatinine Clearance by HPLC	Sharma, Kumar
Creatinine Companion Protocol Assay (Exocell)	Brosius, Frank Leiter, Ed
Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin	Harris, Raymond
Echocardiography: Mouse	Abel, E. Dale
Evaluation of Mitochondrial Function	Abel, E. Dale
Frequently sampled intravenous glucose tolerance test	Clemmons, David Nichols, Timothy
Glucose	McClain, Donald
Glucose uptake in isolated cardiomyocytes.	Abel, E. Dale
Histological Evaluation of Renal Fibrosis in Mice	Takahashi, Takamune
Intraperitoneal Glucose Tolerance Testing (IPGTT)	McClain, Donald
Isoproterenol	Abel, E. Dale
Lipid Profiles in Mice	Breslow, Jan
Lipoprotein separation by FPLC	Breslow, Jan
Measurement of Left Ventricular Hemodynamic Parameters in Intact Mice	Abel, E. Dale
Measurement of Left Ventricular Performance in Langendorff Perfused Mouse Hearts	Abel, E. Dale
Mesangial Index Quantification	Brosius, Frank
Morphometry: Mouse	Leiter, Ed
NADH Oxidase Activity	Feldman, Eva
Nerve Conduction Velocity Tests	Feldman, Eva
Neuropathy Phentoyping Protocols	Feldman, Eva
Pancreatic Insulin Concentration	Leiter, Ed
Phenotypic Characterization of the Working Heart	Abel, E. Dale
Plantar Analgesia Test for Hind Paw and Tail	Feldman, Eva
Plasma Insulin (LINCO ELISA)	Hsueh, Willa
Podocyte Count and Density Analysis	Brosius, Frank
Prepare Plantar Footskin for Morphometry	Calcutt, Nigel
Preparing Nerves for Morphometry	Calcutt, Nigel
Quantification of atherosclerosis at the aortic sinus	Breslow, Jan
Quantitative Magnetization Transfer MR Imaging (qMT-MRI) of Diabetic Mouse Kidney	Takahashi, Takamune
Serum, Blood and Body Weight Measurements in Pigs	Clemmons, David Nichols, Timothy
Tail Flick	Feldman, Eva
Thiobarbituric acid reactive substances	Feldman, Eva
TRAP	Feldman, Eva
Ultra Sensitive Rat Insulin ELISA Kit (Crystal Chem Inc)	Leiter, Ed
Ultracentrifugal separation of HDL alone and calculation of non-HDL	Breslow, Jan
Ultracentrifugal separation of VLDL, LDL and HDL	[Consortial]
Urine Albumin ELISA (Albuwell M kit)	Brosius, Frank Leiter, Ed
Western Analysis used in Oxidative Stress Protocols	Feldman, Eva

Staining	Contact	Complication
aldehyde fuchsin	Leiter, Ed
aldehyde fuchsin/H&E	Feldman, Eva
Fouchet's stain	Leiter, Ed
H&E Staining	[Consortial]
Lead citrate-uranyl acetate	[Consortial]
Masson's trichrome	Leiter, Ed
MOVAT Pentachrome Staining	Alpers, Charles
Periodic acid Schiff (PAS)	Alpers, Charles Leiter, Ed
Silver Methenamine	Alpers, Charles
Toluidine blue	[Consortial]

Technique	Contact	Complication
Determination of Podocyte Number and Density in Rodent Glomeruli	Brosius, Frank
Induce Stroke via Hypoxia-Ischemia	Simpson, Ian
Restoration of euglycemia in the RCS10 mice with Metformin	Simpson, Ian
Vascular perfusion of mice	Harris, Raymond
WT-1 Staining Protocol for Podocytes	Brosius, Frank

Validation Criteria	Contact	Complication
Validation of Mouse Models of Diabetic Cardiovascular Disease	[Consortial]
Validation of Mouse Models of Diabetic Nephropathy	[Consortial]


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