The following protocols are used by the DiaComp. Click the title for details, or download the protocol in PDF format (if available).


Complications:  (Click an image below to filter by diabetic complication)

Cardiovasculary/Cardiomyopathy Cardiovascular
Neuropathy Neuropathy
Wound-Healing Wound-Healing

Gastro-Intestinal Gastro-Intestinal (GI)
Pediatric Endocrinology Pediatric Endocrinology
Retinopathy Retinopathy

Nephropathy Nephropathy
Uropathy Uropathy
All Complications All Complications



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Type: Breeding

 250  Breeding NONcNZO10/LtJ (NON/NZ) and NON/ShiLtJ (NON/Shi) mice1Active       
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 79  Breeding Scheme and Selection of Animals for DCC Experiments1Active     
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Type: Complication Induction

 267  Induction of Type 1 Diabetes, Myocardial Infarction, Echocardiography, Measurement of Blood Glucose and Tissue Weights1Active 
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Type: Diabetes Induction

 221  Ablation of ß-cells in Tg(ins:NTR-mCherry) transgenic zebrafish using metronidazole1Active 
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 220  Dechorionation of zebrafish embryos with Pronase for metronidazole-mediated ß-cell ablation1Active 
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 74  High Dose STZ Induction Protocol1Active       
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 81  Mouse Diets Used in Diabetic Complication Research1Active 
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Type: PCR Amplification

 222  Quantitative PCR analysis to assess gene expression changes in hyperglycemic larval zebrafish1Active 
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Type: Phenotype Assay

 45  Aortic Banding in Mice1Active 
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 49  Atherosclerotic Morphometry in Pigs1Active 
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 56  Cardiac AMPK Assay1Active 
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 38  CATALASE1Active       
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 223  Confocal imaging of live larval zebrafish for assessing peripheral neuropathy1Active 
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 21  Creatinine Clearance by HPLC1Active 
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 23  Creatinine Companion Protocol Assay (Exocell)1Active 
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 28  Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin1Active 
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 50  Frequently sampled intravenous glucose tolerance test1Active     
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 10  Glucose1Active       
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 302  Histological Evaluation of Renal Fibrosis in Mice1Active 
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 11  Intraperitoneal Glucose Tolerance Testing (IPGTT)1Active       
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 58  Isoproterenol1Active 
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 14  Lipoprotein separation by FPLC1Active 
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 43  Measurement of Left Ventricular Hemodynamic Parameters in Intact Mice1Active 
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 64  Mesangial Index Quantification1Active 
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 47  Morphometry: Mouse1Active       
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 36  NADH Oxidase Activity1Active       
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 35  Nerve Conduction Velocity Tests2Active 
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 54  Neuropathy Phentoyping Protocols1Active 
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 73  Pancreatic Insulin Concentration1Active       
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 37  Plantar Analgesia Test for Hind Paw and Tail1Active 
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 48  Plasma Insulin (LINCO ELISA)1Active       
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 65  Podocyte Count and Density Analysis1Active 
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 71  Prepare Plantar Footskin for Morphometry1Active 
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 70  Preparing Nerves for Morphometry1Active 
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 16  Quantification of atherosclerosis at the aortic sinus1Active 
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 303  Quantitative Magnetization Transfer MR Imaging (qMT-MRI) of Diabetic Mouse Kidney1Active 
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 51  Serum, Blood and Body Weight Measurements in Pigs1Active       
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 34  Tail Flick1Active 
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 33  Thiobarbituric acid reactive substances1Active 
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 32  TRAP1Active       
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 29  Ultra Sensitive Rat Insulin ELISA Kit (Crystal Chem Inc)1Active       
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 17  Ultracentrifugal separation of HDL alone and calculation of non-HDL1Active 
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 22  Urine Albumin ELISA (Albuwell M kit)1Active 
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 31  Western Analysis used in Oxidative Stress Protocols1Active 
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Type: Staining

 134  Lead citrate-uranyl acetate1Active      
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 133  Toluidine blue1Active      
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Type: Technique

 251  Induce Stroke via Hypoxia-Ischemia1Active  
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 252  Restoration of euglycemia in the RCS10 mice with Metformin1Active       
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 24  Vascular perfusion of mice1Active     
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Type: Validation Criteria

 26  Validation of Mouse Models of Diabetic Cardiovascular Disease1Active 
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