DiaComp :: Protocols

The following protocols are used by the DiaComp. Click the title for details, or download the protocol in PDF format (if available).

Complications: (Click an image below to filter by diabetic complication)

Cardiovascular

Neuropathy

Wound-Healing

Gastro-Intestinal (GI)

Pediatric Endocrinology

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All Complications

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ID	Title	Version	Active
Type: Breeding
250	Breeding NONcNZO10/LtJ (NON/NZ) and NON/ShiLtJ (NON/Shi) mice	1	Active
79	Breeding Scheme and Selection of Animals for DCC Experiments	1	Active
Type: Complication Induction
267	Induction of Type 1 Diabetes, Myocardial Infarction, Echocardiography, Measurement of Blood Glucose and Tissue Weights	1	Active
Type: Diabetes Induction
221	Ablation of ß-cells in Tg(ins:NTR-mCherry) transgenic zebrafish using metronidazole	1	Active
220	Dechorionation of zebrafish embryos with Pronase for metronidazole-mediated ß-cell ablation	1	Active
74	High Dose STZ Induction Protocol	1	Active
81	Mouse Diets Used in Diabetic Complication Research	1	Active
Type: PCR Amplification
222	Quantitative PCR analysis to assess gene expression changes in hyperglycemic larval zebrafish	1	Active
Type: Phenotype Assay
45	Aortic Banding in Mice	1	Active
49	Atherosclerotic Morphometry in Pigs	1	Active
56	Cardiac AMPK Assay	1	Active
38	CATALASE	1	Active
223	Confocal imaging of live larval zebrafish for assessing peripheral neuropathy	1	Active
21	Creatinine Clearance by HPLC	1	Active
23	Creatinine Companion Protocol Assay (Exocell)	1	Active
28	Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin	1	Active
50	Frequently sampled intravenous glucose tolerance test	1	Active
10	Glucose	1	Active
302	Histological Evaluation of Renal Fibrosis in Mice	1	Active
11	Intraperitoneal Glucose Tolerance Testing (IPGTT)	1	Active
58	Isoproterenol	1	Active
14	Lipoprotein separation by FPLC	1	Active
43	Measurement of Left Ventricular Hemodynamic Parameters in Intact Mice	1	Active
64	Mesangial Index Quantification	1	Active
47	Morphometry: Mouse	1	Active
36	NADH Oxidase Activity	1	Active
35	Nerve Conduction Velocity Tests	2	Active
54	Neuropathy Phentoyping Protocols	1	Active
73	Pancreatic Insulin Concentration	1	Active
37	Plantar Analgesia Test for Hind Paw and Tail	1	Active
48	Plasma Insulin (LINCO ELISA)	1	Active
65	Podocyte Count and Density Analysis	1	Active
71	Prepare Plantar Footskin for Morphometry	1	Active
70	Preparing Nerves for Morphometry	1	Active
16	Quantification of atherosclerosis at the aortic sinus	1	Active
303	Quantitative Magnetization Transfer MR Imaging (qMT-MRI) of Diabetic Mouse Kidney	1	Active
51	Serum, Blood and Body Weight Measurements in Pigs	1	Active
34	Tail Flick	1	Active
33	Thiobarbituric acid reactive substances	1	Active
32	TRAP	1	Active
29	Ultra Sensitive Rat Insulin ELISA Kit (Crystal Chem Inc)	1	Active
17	Ultracentrifugal separation of HDL alone and calculation of non-HDL	1	Active
22	Urine Albumin ELISA (Albuwell M kit)	1	Active
31	Western Analysis used in Oxidative Stress Protocols	1	Active
Type: Staining
134	Lead citrate-uranyl acetate	1	Active
133	Toluidine blue	1	Active
Type: Technique
251	Induce Stroke via Hypoxia-Ischemia	1	Active
252	Restoration of euglycemia in the RCS10 mice with Metformin	1	Active
24	Vascular perfusion of mice	1	Active
Type: Validation Criteria
26	Validation of Mouse Models of Diabetic Cardiovascular Disease	1	Active