A mouse model of simulated birth trauma induced stress urinary incontinence.
Authors Lin YH, Liu G, Daneshgari F
Submitted By Firouz Daneshgari on 2/8/2009
Status Published
Journal Neurourology and urodynamics
Year 2008
Date Published 11/1/2007
Volume : Pages 27(4) : 353 - 358
PubMed Reference 17849481
Abstract AIMS: To facilitate future applications of transgenic or knockout technologies
in studies of simulated birth trauma induced stress urinary incontinence (SUI),
we aimed to create a mouse model of SUI and explore the possible pathogenesis of
this condition. METHODS: Thirty female C57BL/6 mice were randomly distributed
into five groups. Four groups underwent vaginal distention (VD) for 1 hr, using
a modified 6-Fr. Foley catheter with a balloon dilated to 0.3, 0.2, or 0.1 ml or
sham distention. Four days after VD, all mice underwent leak-point pressure
(LPP) measurement via an implanted suprapubic tube (SPT). The normal control
group only had SPT placement and LPP measurement. After sacrifice, the urethras
of the mice were harvested for routine histological examination and nerve
staining. RESULTS: LPPs were significantly lower in groups after VD with 0.3- or
0.2-ml balloon than in control and sham distention groups (10.29 +/- 6.70, 14.65
+/- 6.51, 37.78 +/- 5.10, and 30.30 +/- 5.30 cm H(2)O, respectively). There were
no significant differences in LPP between control and sham groups. Histology
showed no significant differences in urethral striated muscle among the five
groups. The density of immunoreactive neurofilaments in the urethra decreased
after VD with 0.3- or 0.2-ml balloon. CONCLUSION: As a model of birth trauma, VD
can induce SUI in female mice, the severity of which is related to intravaginal
balloon size. Partial urethral denervation plays a plausible role in the
pathogenesis of SUI. This novel mouse model could be used for further
mechanistic studies of female SUI.

Investigators with authorship
Firouz DaneshgariCase Western Reserve