Bladder and urethral function in pelvic organ prolapsed lysyl oxidase like-1
knockout mice.
Authors Liu G, Daneshgari F, Li M, Lin D, Lee U, Li T, Damaser MS
Submitted By Firouz Daneshgari on 2/8/2009
Status Published
Journal BJU international
Year 2007
Date Published 8/1/2007
Volume : Pages 100(2) : 414 - 418
PubMed Reference 17555473
Abstract OBJECTIVES: To examine bladder and urethral function in pelvic organ prolapsed
lysyl oxidase like-1 (LOXL1) knockout mice. MATERIALS AND METHODS: Female parous
Loxl1 (-/-) mice in the stable phase of prolapse, and age-matched wild type (WT)
mice (six each) had conscious cystometry, leak-point pressure (LPP) testing, and
contractile responses assessed of their bladder muscle strips to KCl,
electrical-field stimulation, ATP, and carbachol. RESULTS: Loxl1 (-/-) mice
voided more frequently and had lower mean (sem) bladder capacity, at 0.10 (0.01)
vs 0.20 (0.01) mL, and voiding pressure, at 25.0 (1.90) vs 36.6 (4.04) cmH(2)O,
respectively, during cystometry than had WT mice. The LPP was not significantly
different between WT and Loxl1 (-/-) mice, at 7.05 (0.81) vs 5.22 (1.23)
cmH(2)O, respectively. There were no significant differences between bladder
strips from Loxl1 (-/-) mice and WT mice in their responsiveness to various
stimuli. CONCLUSIONS: Loxl1 (-/-) knockout mice had lower urinary tract
dysfunction, most likely due to urethral dysfunction. Loxl1 (-/-) knockout mice
can be used as an animal model for pelvic floor disorders. Further studies are
needed to characterize the morphological and molecular alterations of the
bladder and urethra.


Investigators with authorship
NameInstitution
Firouz DaneshgariCase Western Reserve

Complications









Genes
SymbolDescription
Pax1paired box gene 1
Wtwaltzer-type
Loxl1lysyl oxidase-like 1