Considerations for quantification of lipids in nerve tissue using
matrix-assisted laser desorption/ionization mass spectrometric imaging.
Authors Landgraf RR, Garrett TJ, Conaway MC, Calcutt NA, Stacpoole PW, Yost RA
Submitted By Nigel Calcutt on 1/30/2013
Status Published
Journal Rapid communications in mass spectrometry : RCM
Year 2011
Date Published 10/30/2011
Volume : Pages 25 : 3178 - 3184
PubMed Reference 21953974
Abstract Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging
is a technique that provides the ability to identify and characterize endogenous
and exogenous compounds spatially within tissue with relatively little sample
preparation. While it is a proven methodology for qualitative analysis, little
has been reported for its utility in quantitative measurements. In the current
work, inherent challenges in MALDI quantification are addressed. Signal response
is monitored over successive analyses of a single tissue section to minimize
error due to variability in the laser, matrix application, and sample
inhomogeneity. Methods for the application of an internal standard to tissue
sections are evaluated and used to quantify endogenous lipids in nerve tissue. A
precision of 5% or less standard error was achieved, illustrating that MALDI
imaging offers a reliable means of in situ quantification for microgram-sized
samples and requires minimal sample preparation.

Investigators with authorship
Nigel CalcuttUniversity of California San Diego