||Perez-Diaz S, Johnson LA, DeKroon RM, Moreno-Navarrete JM, Alzate O,
Fernandez-Real JM, Maeda N, Arbones-Mainar JM
||Submitted Externally on 8/5/2014
||FASEB journal : official publication of the Federation of American Societies for Experimental Biology
|Volume : Pages
||28 : 3769 - 3779
||Impaired adipogenesis renders an adipose tissue unable to expand, leading to
lipotoxicity and conditions such as diabetes and cardiovascular disease. While
factors important for adipogenesis have been studied extensively, those that set
the limits of adipose tissue expansion remain undetermined. Feeding a
Western-type diet to apolipoprotein E2 knock-in mice, a model of metabolic
syndrome, produced 3 groups of equally obese mice: mice with normal glucose
tolerance, hyperinsulinemic yet glucose-tolerant mice, and prediabetic mice with
impaired glucose tolerance and reduced circulating insulin. Using proteomics, we
compared subcutaneous adipose tissues from mice in these groups and found that
the expression of PTRF (polymerase I and transcript release factor) associated
selectively with their glucose tolerance status. Lentiviral and
pharmacologically overexpressed PTRF, whose function is critical for caveola
formation, compromised adipocyte differentiation of cultured 3T3-L1cells. In
human adipose tissue, PTRF mRNA levels positively correlated with markers of
lipolysis and cellular senescence. Furthermore, a negative relationship between
telomere length and PTRF mRNA levels was observed in human subcutaneous fat.
PTRF is associated with limited adipose tissue expansion underpinning the key
role of caveolae in adipocyte regulation. Furthermore, PTRF may be a suitable
adipocyte marker for predicting pathological obesity and inform clinical
management.-Perez-Diaz, S., Johnson, L. A., DeKroon, R. M., Moreno-Navarrete, J.
M., Alzate, O., Fernandez-Real, J. M., Maeda, N., Arbones-Mainar, J. M.
Polymerase I and transcript release factor (PTRF) regulates adipocyte
differentiation and determines adipose tissue expandability.