Ventral prostate fibrosis in the Akita mouse is associated with macrophage and
fibrocyte infiltration.
Authors Lee S, Yang G, Mulligan W, Gipp J, Bushman W
Submitted By Wade Bushman on 8/5/2014
Status Published
Journal Journal of diabetes research
Year 2014
Date Published
Volume : Pages 2014 : 939053
PubMed Reference 25019092
Abstract A higher incidence of lower urinary tract symptoms (LUTS) among diabetic men is
unexplained. Recently, prostate inflammation and fibrosis have been implicated
as major contributing factors to bladder outlet obstruction and LUTS. We
characterized the inflammatory cell infiltrate and collagen content of the
anterior, dorsal, and ventral lobes of 18-week-old DBA2J.Ins2-Akita mice (Akita)
and age-matched control mice. We performed hematoxylin and eosin staining to
score tissue injury and inflammation, picrosirius red staining to quantitate
collagen content, and immunostaining to identify monocytes/macrophages and
infiltrating fibrocytes. We observed significantly greater numbers of
monocytes/macrophages and fibrocytes specifically in the ventral prostate of the
Akita mice and found that this was associated with significant greater collagen
content specifically in the ventral prostate of the Akita mice. These
observations support the inference that diabetes elicits monocyte/macrophage
infiltration and collagen accumulation in the prostate and suggest that further
study of Akita mice may inform translational studies of diabetes in the genesis
prostatic inflammation, prostatic fibrosis, and LUTS.


Investigators with authorship
NameInstitution
Wade BushmanUniversity of Wisconsin-Madison

Complications