Impaired cytokine expression, neutrophil infiltration, and bacterial clearance
in response to urinary tract infection in diabetic mice.
Authors Ozer A, Altuntas CZ, Bicer F, Izgi K, Hultgren SJ, Liu G, Daneshgari F
Submitted By Firouz Daneshgari on 3/9/2015
Status Published
Journal Pathogens and disease
Year 2015
Date Published 2/5/2015
Volume : Pages Not Specified : Not Specified
PubMed Reference 25663347
Abstract Diabetic patients have increased susceptibility to infections, and urinary tract
infections (UTI) are the most common type in women with diabetes mellitus.
Knowledge of bacterial clearance effectiveness following UTI in diabetics is
sparse. In this study, the effects of diabetes on bacterial clearance efficiency
and components of the innate immune system in response to UTI in a murine model
were investigated. Streptozotocin-induced diabetic and control female C57BL/6J
mice were infected with uropathogenic Escherichia coli, and bacterial load,
expression of chemokines, and neutrophil infiltration in the bladder over time
were investigated. Expression levels of histone deacetylases were also measured
to address a potential mechanism underlying the phenotype. Bacterial clearance
during UTI was significantly prolonged in diabetic mice relative to controls.
Neutrophil infiltration in bladder tissue and urine, and both mRNA and protein
expression of chemokines MIP-2, KC, MCP-1, and IL-6 in bladder tissue were
diminished at early time points after infection in diabetic mice relative to
controls. In addition, mRNA levels of histone deacetylases 1-5 were increased in
diabetic mice. This is the first study to show an association of impaired
bacterial clearance in diabetic mice with suppression of UTI-induced chemokine
expression and neutrophil infiltration in the bladder.

Investigators with authorship
Firouz DaneshgariCase Western Reserve
Guiming LiuCase Western Reserve