The effects of anesthesia on measures of nerve conduction velocity in male
C57Bl6/J mice.
Authors Oh SS, Hayes JM, Sims-Robinson C, Sullivan KA, Feldman EL
Submitted By Eva Feldman on 3/4/2010
Status Published
Journal Neuroscience letters
Year 2010
Date Published 10/11/2010
Volume : Pages 483 : 127 - 131
PubMed Reference 20691755
Abstract Animal models, particularly mice, are used extensively to investigate
neurological diseases. Basic research regarding animal models of human
neurological disease requires that the animals exhibit hall mark characteristics
of the disease. These include disease specific anatomical, metabolic and
behavioral changes. Nerve conduction velocity (NCV) is the predominant method
used to assess peripheral nerve health. Normative data adjusted for age, gender
and height is available for human patients; however, these data are not
available for most rodents including mice. NCV may be affected by animal age and
size, body temperature, stimulus strength and anesthesia. While the effects of
temperature, age and size are documented, the direct and indirect effects of
anesthesia on NCV are not well reported. Our laboratory is primarily concerned
with animal models of diabetic neuropathy (DN) and uses NCV to confirm the
presence of neuropathy. To ensure that subtle changes in NCV are reliably
assayed and not directly or indirectly affected by anesthesia, we compared the
effects of 4 commonly used anesthetics, isoflurane, ketamine/xylazine, sodium
pentobarbital and 2-2-2 tribromoethanol on NCV in a commonly used rodent model,
the C57Bl6/J mouse. Our results indicate that of the anesthetics tested,
isoflurane has minimal impact on NCV and is the safest, most effective method of
anesthesia. Our data strongly suggest that isoflurane should become the
anesthetic of choice when performing NCV on murine models of neurological

Investigators with authorship
Eva FeldmanUniversity of Michigan