Functional and morphological alterations of the urinary bladder in type 2
diabetic FVB(db/db) mice.
Authors Wu L, Zhang X, Xiao N, Huang Y, Kavran M, Elrashidy RA, Wang M, Daneshgari F,
Liu G
Submitted By Guiming Liu on 4/4/2016
Status Published
Journal Journal of diabetes and its complications
Year 2016
Date Published 3/1/2016
Volume : Pages Not Specified : Not Specified
PubMed Reference 27037041
Abstract Diabetic bladder dysfunction (DBD) has been extensively studied in animal models
of type 1 diabetes. We aimed to examine the functional and morphological
alterations of the urinary bladder in a type 2 diabetes model, FVB(db/db) mice.,
FVB(db/db) mice and age-matched FVB/NJ control mice were tested at either 12, 24
or 52weeks of age. Body weight, blood glucose and glycated hemoglobin (HbA1c)
levels were measured. Bladder function was assessed by measurement of 24-h
urination behavior and conscious cystometry. Bladder was harvested for Masson's
Trichrome staining and morphometric analysis., The body weights of FVB(db/db)
mice were twice as those of FVB/NJ control mice. The blood glucose and HbA1c
levels were higher in FVB(db/db) mice at 12 and 24weeks, but not at 52weeks. A
significant increase in the mean volume per void, but decrease in the voiding
frequency, in FVB(db/db) mice was observed. Cystometry evaluation showed
increased bladder capacity, voided volume, and peak micturition pressure in
FVB(db/db) mice compared with FVB/NJ mice. Morphometric analysis revealed a
significant increase in the areas of detrusor muscle and urothelium in
FVB(db/db) mice. In addition, some FVB(db/db) mice, especially males at 12 and
24weeks, showed small-volume voiding during 24-h urination behavior measurement,
and detrusor overactivity in the cystometry measurement., The FVB(db/db) mouse,
displaying DBD characterized by not only increased bladder capacity, void
volume, and micturition pressure, but also bladder overactivity, is a useful
model to further investigate the mechanisms of type 2 diabetes-related bladder