Voiding function in obese and type 2 diabetic female rats.
Authors Gasbarro G, Lin DL, Vurbic D, Quisno A, Kinley B, Daneshgari F, Damaser MS
Submitted By Firouz Daneshgari on 3/31/2010
Status Published
Journal American journal of physiology. Renal physiology
Year 2010
Date Published 1/1/2010
Volume : Pages 298 : F72 - F77
PubMed Reference 19889955
Abstract The effects of obesity and type 2 diabetes (DMII) on the lower urinary tract
(LUT) were characterized by evaluating voiding function and anatomy in female
Zucker diabetic fatty (ZDF) rats. Age-matched female virgin rats were separated
into three experimental groups: Zucker lean rats (control; normal diet, n = 22),
ZDF rats (obese+nondiabetic; low-fat diet, n = 22), and ZDF rats
(obese+diabetic; high-fat diet, n = 20). Rats were placed on their specified
diet for 10 wk before urodynamic LUT evaluation. A suprapubic catheter was
implanted 2 days before urodynamic studies. Voiding function was evaluated by
cystometric and leak point pressure (LPP) testing. The bladder, urethra, and
vagina were immediately excised for qualitative histological evaluation.
Compared with control rats, obese+nondiabetic and obese+diabetic rats had
significantly decreased contraction pressure (P = 0.003) and increased
cystometric filling volume (P < 0.001). Both obese groups exhibited
significantly higher voided volumes (P = 0.003), less frequent urinary events (P
< 0.001), and increased residual volumes (P = 0.039). LPP studies showed a
nonsignificant decrease in LPP (P = 0.075) and baseline pressure (P = 0.168) in
both obese groups compared with control. Histology of the external urethral
sphincter in obese rats showed increased fibrosis, leading to disruption of the
skeletal muscle structure compared with control. Additionally, the bladder wall
of the obese+nondiabetic and obese+diabetic rats demonstrated edema and
vasculopathy. Voiding dysfunction was evident in both obese groups but with no
significant differences due to DMII, suggesting that voiding dysfunction in DMII
may be attributable at least in part to chronic obesity.

Investigators with authorship
Firouz DaneshgariCase Western Reserve