Efficacy of a novel device for assessment of autonomic sensory function in the
rat bladder.
Authors Abouassaly R, Liu G, Yamada Y, Ukimura O, Daneshgari F
Submitted By Firouz Daneshgari on 3/31/2010
Status Published
Journal The Journal of urology
Year 2008
Date Published 3/1/2008
Volume : Pages 179 : 1167 - 1172
PubMed Reference 18206176
Abstract PURPOSE: We developed and tested the efficacy of an implantable bladder device
which, when combined with the Neurometer, can be used to assess fiber specific
afferent bladder sensation in the rat. MATERIALS AND METHODS: We developed an
implantable bladder device that applies selective nerve fiber stimuli (250 Hz
for small myelinated Adelta fibers and 5 Hz for unmyelinated C fibers) to the
bladder mucosa in the rat to determine bladder sensory perception threshold
values. We performed 3 experiments in 55 female Sprague-Dawley rats to examine
the effects of our device on voiding habits, assess the interobserver
reliability of the sensory perception threshold and determine the effects of
intravesical administration of resiniferatoxin (Sigma) and lidocaine on the
sensory perception threshold. RESULTS: Sensory perception threshold values
obtained by 2 blinded, independent observers were not different from each other
(p = 0.41). Sensory perception threshold values obtained at the 2 stimulation
frequencies remained constant for at least 3 weeks after device implantation. A
significant increase in sensory perception threshold values after
resiniferatoxin instillation was noted at a stimulus frequency of 5 Hz (p =
0.02), whereas intravesical lidocaine led to an immediate increase in the
sensory perception threshold at 250 and 5 Hz. Device implantation led to an
early decreased voided volume and increased frequency of voids, although these
parameters returned to normal after 4 days. CONCLUSIONS: Assessment of bladder
afferent sensation with our newly developed device is feasible in rats. It
provides sensory perception thresholds that appear to be fiber-type selective
for autonomic bladder afferent nerves.

Investigators with authorship
Firouz DaneshgariCase Western Reserve