Aging and diabetes impair the neovascular potential of adipose-derived stromal
Authors El-Ftesi S, Chang EI, Longaker MT, Gurtner GC
Submitted By Geoffrey Gurtner on 3/31/2010
Status Published
Journal Plastic and reconstructive surgery
Year 2009
Date Published 2/1/2009
Volume : Pages 123 : 475 - 485
PubMed Reference 19182604
Abstract BACKGROUND: Aging and diabetes are major risk factors for poor wound healing and
tissue regeneration that reflect an impaired ability to respond to ischemic
insults. The authors explored the intrinsic neovascular potential of
adipose-derived stromal cells in the setting of advanced age and in type 1 and
type 2 diabetes. METHODS: Adipose-derived stromal cells isolated from young,
aged, streptozotocin-induced, and db/db diabetic mice were exposed to normoxia
and hypoxia in vitro. Vascular endothelial growth factor (VEGF) expression,
proliferation, and tubulization were measured. Conditioned media harvested from
adipose-derived stromal cell cultures were assessed for their ability to
stimulate human umbilical vein endothelial cell proliferation (n = 3 and n = 3).
RESULTS: Young adipose-derived stromal cells demonstrated significantly higher
levels of VEGF production, proliferation, and tubulogenesis than those derived
from aged, streptozotocin-induced, and db/db mice in both normoxia and hypoxia.
Although aged and diabetic adipose-derived stromal cells retained the ability to
up-regulate VEGF secretion, proliferation, and tubulogenesis in response to
hypoxia, the response was blunted compared with young controls. Conditioned
media derived from these cells cultured in normoxia in vitro also had a
significantly greater ability to increase human umbilical vein endothelial cell
proliferation compared with media harvested from aged, streptozotocin-induced,
and db/db adipose-derived stromal cells. This effect was magnified in
conditioned media harvested from hypoxic adipose-derived stromal cell cultures.
CONCLUSIONS: This study demonstrates that aging and type 1 and type 2 diabetes
impair intrinsic adipose-derived stromal cell function; however, these cells may
still be a suitable source of angiogenic cells that can potentially improve
neovascularization of ischemic tissues.

Investigators with authorship
Geoffrey GurtnerStanford University


Vegfavascular endothelial growth factor A