Laser capture microdissection of kidney tissue.
Authors Woroniecki RP, Bottinger EP
Submitted By Erwin Bottinger on 5/2/2011
Status Published
Journal Methods in molecular biology (Clifton, N.J.)
Year 2009
Date Published 3/20/2009
Volume : Pages 466 : 73 - 82
PubMed Reference 19148600
Abstract Kidney tissue laser capture microdissection (LCM) is of great clinical relevance
since genome wide studies on total kidney messenger RNA (mRNA) potentially miss
important factors involved in the pathogenesis of the disease in glomeruli and
tubules. This technique is readily applicable to study mRNA from isolated
glomeruli and tubules of human kidney biopsy material. In this chapter we
present a "cook-book" practical approach of utilizing LCM in combination with
RNA isolation technique in downstream applications in nephrology.

Investigators with authorship
Erwin BottingerMount Sinai School of Medicine