Quantitative Large-Scale Three-Dimensional Imaging of Human Kidney Biopsies: A
Bridge to Precision Medicine in Kidney Disease.
Authors Winfree S, Dagher PC, Dunn KW, Eadon MT, Ferkowicz M, Barwinska D, Kelly KJ,
Sutton TA, El-Achkar TM
Submitted By Pierre Dagher on 6/18/2018
Status Published
Journal Nephron
Year 2018
Date Published
Volume : Pages Not Specified : 1 - 6
PubMed Reference 29870980
Abstract Kidney biopsy remains the gold standard for uncovering the pathogenesis of acute
and chronic kidney diseases. However, the ability to perform high resolution,
quantitative, molecular and cellular interrogation of this precious tissue is
still at a developing stage compared to other fields such as oncology. Here, we
discuss recent advances in performing large-scale, three-dimensional (3D),
multi-fluorescence imaging of kidney biopsies and quantitative analysis referred
to as 3D tissue cytometry. This approach allows the accurate measurement of
specific cell types and their spatial distribution in a thick section spanning
the entire length of the biopsy. By uncovering specific disease signatures,
including rare occurrences, and linking them to the biology in situ, this
approach will enhance our understanding of disease pathogenesis. Furthermore, by
providing accurate quantitation of cellular events, 3D cytometry may improve the
accuracy of prognosticating the clinical course and response to therapy.
Therefore, large-scale 3D imaging and cytometry of kidney biopsy is poised to
become a bridge towards personalized medicine for patients with kidney disease.

Investigators with authorship
Pierre DagherIndiana University - Indianapolis