Sign-up for our newsletter
MAIN
Event Calendar
Awardee Reports
ABOUT DIACOMP
Citing DiaComp
Contact
Committees
Institutions
Awardee Reports
Publications
Bioinformatics
RESOURCES
Protocols & Methods
Reagents & Resources
Mouse Diet
Breeding Schemes
Validation Criteria
IMPC / KOMP Data
Publications
Bioinformatics
CONTACT
PARTICIPANT AREA
Login
▹
Publications
▹
Home
Publication
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion
Microscopy.
Authors
Chozinski TJ, Mao C, Halpern AR, Pippin JW, Shankland SJ, Alpers CE, Najafian B,
Vaughan JC
Submitted By
Joshua Vaughan on 7/30/2018
Status
Published
Journal
Scientific reports
Year
2018
Date Published
Volume : Pages
8 : 10396
PubMed Reference
29991751
Abstract
Although light microscopy is a powerful tool for the assessment of kidney
physiology and pathology, it has traditionally been unable to resolve structures
separated by less than the ~250?nm diffraction limit of visible light. Here, we
report on the optimization, validation, and application of a recently developed
super-resolution fluorescence microscopy method, called expansion microscopy
(ExM), for volumetric interrogation of mouse and human kidney tissue with
70-75?nm lateral and ~250?nm axial spatial resolution. Using ExM with a standard
confocal microscope, we resolve fine details of structures that have
traditionally required visualization by electron microscopy, including podocyte
foot processes, the glomerular basement membrane, and the cytoskeleton. This
inexpensive and accessible approach to volumetric, nanoscale imaging enables
visualization of fine structural details of kidney tissues that were previously
difficult or impossible to measure by conventional methodologies.
Investigators with authorship
Name
Institution
Charles Alpers
University of Washington
Joshua Vaughan
University of Washington
Complications
All Complications
Bioinformatics
Bone
Cardiomyopathy
Cardiovascular
Gastro-Intestinal (GI)
Nephropathy
Neuropathy & Neurocognition
Pediatric Endocrinology
Retinopathy
Uropathy
Wound Healing
Welcome to the DiaComp Login / Account Request Page.
Email Address:
Password:
Note: Passwords are case-sensitive.
Please save my Email Address on this machine.
Not a member?
If you are a funded DiaComp investigator, a member of an investigator's lab,
or an External Scientific Panel member to the consortium, please
request an account.
Forgot your password?
Enter your Email Address and
click here.
ERROR!
There was a problem with the page:
User Info
User Confirm
Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
Citation text and image have been copied to your clipboard. You may now paste them into your document. Thank you!