Genome assembly and gene expression in the American black bear provides new
insights into the renal response to hibernation.
Authors Srivastava A, Kumar Sarsani V, Fiddes I, Sheehan SM, Seger RL, Barter ME,
Neptune-Bear S, Lindqvist C, Korstanje R
Submitted By Ron Korstanje on 11/6/2018
Status Published
Journal DNA research : an international journal for rapid publication of reports on genes and genomes
Year 2019
Date Published 2/1/2019
Volume : Pages 26 : 37 - 44
PubMed Reference 30395234
Abstract The prevalence of chronic kidney disease (CKD) is rising worldwide and 10-15% of
the global population currently suffers from CKD and its complications. Given
the increasing prevalence of CKD there is an urgent need to find novel treatment
options. The American black bear (Ursus americanus) copes with months of lowered
kidney function and metabolism during hibernation without the devastating
effects on metabolism and other consequences observed in humans. In a biomimetic
approach to better understand kidney adaptations and physiology in hibernating
black bears, we established a high-quality genome assembly. Subsequent RNA-Seq
analysis of kidneys comparing gene expression profiles in black bears entering
(late fall) and emerging (early spring) from hibernation identified 169
protein-coding genes that were differentially expressed. Of these, 101 genes
were downregulated and 68 genes were upregulated after hibernation. Fold changes
ranged from 1.8-fold downregulation (RTN4RL2) to 2.4-fold upregulation (CISH).
Most notable was the upregulation of cytokine suppression genes (SOCS2, CISH,
and SERPINC1) and the lack of increased expression of cytokines and genes
involved in inflammation. The identification of these differences in gene
expression in the black bear kidney may provide new insights in the prevention
and treatment of CKD.

Investigators with authorship
Ron KorstanjeJackson Laboratory