Microscopy with ultraviolet surface excitation for wide-area pathology of breast
surgical margins.
Authors Xie W, Chen Y, Wang Y, Wei L, Yin C, Glaser AK, Fauver ME, Seibel EJ, Dintzis
SM, Vaughan JC, Reder NP, Liu JTC
Submitted By Submitted Externally on 2/28/2019
Status Published
Journal Journal of biomedical optics
Year 2019
Date Published 2/1/2019
Volume : Pages 24 : 1 - 11
PubMed Reference 30737911
Abstract Intraoperative assessment of breast surgical margins will be of value for
reducing the rate of re-excision surgeries for lumpectomy patients. While
frozen-section histology is used for intraoperative guidance of certain cancers,
it provides limited sampling of the margin surface (typically <1??%?? of the
margin) and is inferior to gold-standard histology, especially for fatty tissues
that do not freeze well, such as breast specimens. Microscopy with ultraviolet
surface excitation (MUSE) is a nondestructive superficial optical-sectioning
technique that has the potential to enable rapid, high-resolution examination of
excised margin surfaces. Here, a MUSE system is developed with fully automated
sample translation to image fresh tissue surfaces over large areas and at
multiple levels of defocus, at a rate of ~5??min??/??cm2. Surface extraction is
used to improve the comprehensiveness of surface imaging, and 3-D deconvolution
is used to improve resolution and contrast. In addition, an improved fluorescent
analog of conventional H&E staining is developed to label fresh tissues within
~5??min for MUSE imaging. We compare the image quality of our MUSE system with
both frozen-section and conventional H&E histology, demonstrating the
feasibility to provide microscopic visualization of breast margin surfaces at
speeds that are relevant for intraoperative use.