| Species |
M. musculus |
| Official Name |
B6.Cg-Ins2Akita Ldlrtm1Her/J |
| Common Name |
B6.Cg-Ins2Akita Ldlrtm1Her/J |
| Description |
Mice homozygous for the Akita spontaneous mutation die
postnatally, typically by 12 weeks of age. Independently,
heterozygous Akita mutant mice are a model of insulin
dependent diabetes mellitus (IDDM) with severe hyperglycemia
(see the datasheet for Stock No. 003548 for additional
information). LDLR-null homozygotes have elevated serum
cholesterol levels (200-400 mg/dl) which can escalate to
very high levels (> 2000 mg/dl) when the mice are fed a high
fat diet. LDLR-deficient mice also are predisposed to
develop atherosclerosis. These double mutant mice may be
useful in studies of diabetes, metabolism, hyperglycemia,
atherosclerosis, hypercholesterolemia, and diabetes-related
macrovascular complications.
|
| Development Status |
Characterizing multiple lines |
| Creation Method |
chemical induced |
| Phenotype Description |
The Ldlrtm1Her mutation was made by Dr. Robert Hammer and
Joachim Herz (HHMI, University of Texas Southwestern Medical
Center). Briefly, a targeting vector was used to insert a
neo cassette into exon 4. The vector was electroporated into
129S7/SvEvBrd-derived AB1 embryonic stem (ES) cells.
Chimeric mice were bred to C57BL/6J, and the strain was made
congenic on a C57BL/6J genetic background at The Jackson
Laboratory (Stock No. 002207). The Akita spontaneous
mutation in the insulin II gene (originally called Mody4)
was identified on the C57BL/6NJcl genetic background by Dr.
Akio Koizumi (The Akita University School of Medicine).
These mice were shipped to The Jackson Laboratory and then
made congenic on the C57BL/6J genetic background (Stock No.
003548). To generate the double mutant strain, Stock No.
002207 mice were bred with Stock No. 003548 mice in the
laboratory of Dr. Jan Breslow at The Rockefeller University.
Double mutant mice were then backcrossed to C57BL/6J for
approximately 7 generations prior to arrival at The Jackson
Laboratory. The donating investigators indicate that 103/104
microsatellite markers indicate C57BL/6J background. The
single exception is D7Mit81.
|