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University of Iowa
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Development of an off-the-shelf injectable MSC Microcarrier for Diabetic Wound Healing
Despite advances in wound care including bioengineered skin substitutes and negative pressure wound therapy, 1 in 4 patients with diabetic foot ulcers are likely to face amputation. Mesenchymal stem cell (MSC)-based therapies have shown promise in accelerating healing in proof-of-concept studies. However, past strategies have required on site cell culture facilities in order to prepare the MSC treatment. Our objective in this DiaComp Pilot and Feasibility application is to provide proof of concept for an off-the-shelf delivery method to enhance the viability of transplanted MSCs while maintaining their angiogenic and anti-inflammatory function. Our central hypothesis is that microcarriers will provide an effective cryopreservable microniche for MSCs resulting in improved survival of the cells as compared to those delivered in suspension and comparable function to those delivered without cryopreservation. We have recently developed a strategy to generate biodegradable ~100-400 µm diameter injectable microcarriers, each serving as an attachment point for hundreds of cells. The microcarriers have been designed to be biodegradable and able to withstand cryopreservation after seeding with MSCs. We expect delivery of MSCs on biodegradable injectable microcarriers to enhance MSCs potential to promote healing of diabetic wounds. The rationale underlying the proposed research is that by developing an off-the-shelf system for MSC delivery we will remove a significant hurdle to the widespread adoption of cell-therapy for diabetic wound healing. We plan the following specific aims: 1) Tune biodegradable injectable microcarriers to optimize cell loading, viability, function, and recovery after cryopreservation. 2) Pilot study to determine the ability of cryopreserved microcarriers to accelerate healing in an excisional diabetic wound model. This work will provide the basis for future R01 applications examining MSC microcarrier therapy in a mouse model of diabetic wounds and elucidation of mechanisms utilized by MSCs in vivo to promote healing.
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Development of an off-the-shelf injectable MSC Microcarrier for Diabetic Wound Healing (Ankrum, James)
View Progress Report Document
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Year: 2017; Items: 1
IFN-g and TNF-a Pre-licensing Protects Mesenchymal Stromal Cells from the Pro-inflammatory Effects of Palmitate.
Boland L, Burand AJ, Brown AJ, Boyt D, Lira VA, Ankrum JA
Molecular therapy : the journal of the American Society of Gene Therapy
Year: 2016; Items: 1
Function of Cryopreserved Mesenchymal Stromal Cells With and Without Interferon-? Prelicensing is Context Dependent.
Burand AJ, Gramlich OW, Brown AJ, Ankrum JA
Stem cells (Dayton, Ohio)
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No protocols found.
The DiaComp Steering Committee is the governing body of the consortium. The principle function of this committee is to guide the scientific direction of the consortium. This is accomplished by creating various subcommittees necessary to advance the scientific goals and providing guidance to the broader complications research community. Policies for the consortium are developed through consultation with the
External Evaluation Committee
The DiaComp Wound Healing Committee has the principal function of furthering the mission of the consortium with regard to diabetic wound healing.
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Please acknowledge all posters, manuscripts or scientific materials that were generated in part or whole using funds from the Diabetic Complications Consortium(DiaComp) using the following text:
Financial support for this work provided by the NIDDK Diabetic Complications Consortium (RRID:SCR_001415, www.diacomp.org), grants DK076169 and DK115255
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