| Description |
Insertion of a neomycin resistance cassette into exon 4. The
authors predict that the targeted allele would encode a
truncated non-functional protein that will not bind LDL, and
that lacks a membrane spanning segment. Immunoblot analysis
of liver membranes detected a truncated protein in
homozygous mutant animals.
Mice homozygous for the Ldlrtm1Her mutation have an elevated
serum cholesterol level of 200-400 mg/dl and they have very
high levels (>2,000 mg/dl) when fed a high fat diet. Normal
serum cholesterol in the mouse is 80-100 mg/dl.
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| Phenotype Description |
Strain Development
The Ldlrtm1Her mutant strain was developed in the laboratory
of Dr. Robert Hammer and Dr. Joachim Herz at the Howard
Hughes Medical Institute Research Laboratories, University
of Texas Southwestern Medical Center at Dallas. The
129-derived AB1 ES cell line was used. C57BL/6J mice were
generated by crossing the Ldlrtm1Her mutation 5 or more
times to C57BL/6J mice.
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